What is HPLC testing?
High-performance liquid chromatography (HPLC) is the standard analytical technique for verifying research peptide purity. A dissolved sample is pushed through a chromatographic column; the resulting UV absorbance trace shows the target peptide as a peak alongside any impurities.
How HPLC purity is reported
Purity is expressed as a percentage of total peak area at a specified UV wavelength (typically 214 or 220 nm). Research-grade peptides target ≥99%.
Analytical vs preparative HPLC
Preparative HPLC purifies bulk peptide during manufacturing. Analytical HPLC is the QC step that certifies the finished batch — the trace behind the Certificate of Analysis.
What HPLC does not tell you
HPLC confirms purity but not identity. That is why we pair it with mass spectrometry — see Understanding COAs for peptides and Peptide purity standards.
Disclaimer
For laboratory research use only. Not for human or veterinary use, diagnosis, treatment, cure, or prevention of any disease.